african green monkey kidney epithelial cells vero  (ATCC)

Journal: Vaccines

Article Title: Establishment and Validation of a Cell-Based Relative Potency Method for Respiratory Syncytial Virus mRNA Vaccine Drug Substance

doi: 10.3390/vaccines14050401

Figure Lengend Snippet: Optimization of cell-related conditions for the RSV mRNA potency assay. ( A ) Dose–response curves of RSV mRNA transfection in different cell lines (293T, Vero, HEp-2, HepG2, and A549). Cells were transfected with increasing amounts of RSV mRNA (0.0002–0.48 µg/well), and RSV prefusion F protein expression was measured by ELISA as OD 450 –OD 630 . The corresponding EC 50 and slope values are summarized in the inset table. ( B ) Effect of HepG2 cell seeding density on RSV prefusion F protein expression. HepG2 cells were seeded at 0.5 × 10 5 , 1.0 × 10 5 , or 2.0 × 10 5 cells/well and transfected with increasing amounts of RSV mRNA (0.0002–0.48 µg/well). Protein expression was measured by ELISA as OD 450 –OD 630 .

Article Snippet: Cell lines used for mRNA transfection included the human liver carcinoma cell line HepG2 (American Type Culture Collection, ATCC, Manassas, VA, USA), human non-small cell lung carcinoma cell line A549 (ATCC), African green monkey kidney epithelial cell line Vero (ATCC), human epidermoid carcinoma type 2 cell line Hep-2 (ATCC), and human embryonic kidney cell line 293T (China Center for Type Culture Collection, CCTCC, Wuhan, China).

Techniques: Potency Assay, Transfection, Expressing, Enzyme-linked Immunosorbent Assay

Journal: PLOS Pathogens

Article Title: Entry, replication and innate immunity evasion of BANAL-236, a SARS-CoV-2-related bat virus, in Rhinolophus and human cells

doi: 10.1371/journal.ppat.1013573

Figure Lengend Snippet: (A-P) Caco-2 and RFe-ATC cells were infected for 72 h. Caco-2 cells were infected with SARS-CoV-2 (A-D) or BANAL-236 (I-L) at an MOI 0.001 and 0.01 respectively. RFe-ATC cells were infected with SARS-CoV-2 (E-H) and BANAL-236 ( M-P ) at MOI 0.02 and 0.2 respectively. Cells were then subjected to transmission electron microscopy analysis. DMV-like structures are pointed by white arrows. (Q-R) At 48 and 72 h post-infection supernatants from Caco-2 (Q) and RFe-ATC (R) cells were collected. Titration of clarified supernatants by TCID 50 assays was performed on Vero-E6 cells infected for 5 days. Data are the means ± SEM of at least two independent experiments.

Article Snippet: Human colorectal adenocarcinoma Caco-2 cells (kind gift from Nathalie Sauvonnet, Institut Pasteur, Paris), African green monkey kidney epithelial Vero-E6 cells (ATCC CRL-1586), A549 lung cancer cells (ATCC CCL-185), and human embryonic kidney (HEK) 293T cells (ATCC CRL-3216), hereinafter referred to as 293T, were maintained in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco) containing GlutaMAX I, sodium pyruvate (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (FBS) (Dutscher) and 1% penicillin and streptomycin (10 000 IU/ml; Thermo Fisher Scientific).

Techniques: Infection, Transmission Assay, Electron Microscopy, Titration